pTRIP-SFFV-mtagBFP-2A STING

LOCUS       pTRIP-SFFV-mtagBFP-2A STING               11496 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Lentivector to express STING and mTagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    pTRIP-SFFV-mtagBFP-2A STING
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg 
            MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
            N
  TITLE     Intrinsic antiproliferative activity of the innate sensor STING in T
            lymphocytes.
  JOURNAL   J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674. 
            Epub 2017 May 8.
  PUBMED    28484079
REFERENCE   2  (bases 1 to 11496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11496
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             5900..6080
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7608..7627)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7841..7860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7960..7982
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8020..8038)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8106..8210
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8211..9071
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8429..8448)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9242..9830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9731..9750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9984..10001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10071..10091)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10076..10405
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10256..10391
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10318..10337
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3781 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3901 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     4201 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
     4261 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc catgccccac
     4501 tccagcctgc atccatccat cccgtgtccc aggggtcacg gggcccagaa ggcagccttg
     4561 gttctgctga gtgcctgcct ggtgaccctt tgggggctag gagagccacc agagcacact
     4621 ctccggtacc tggtcctcca cctagcctcc ctgcagctgg gactgctgtt aaacggggtc
     4681 tgcagcctgg ctgaggagct gcgccacatc cactccaggt accggggcag ctactggagg
     4741 actgtgcggg cctgcctggg ctgccccctc cgccgtgggg ccctgttgct gctgtccatc
     4801 tatttctact actccctccc aaatgcggtc ggcccgccct tcacttggat gcttgccctc
     4861 ctgggcctct cgcaggcact gaacatcctc ctgggcctca agggcctggc cccagctgag
     4921 atctctgcag tgtgtgaaaa agggaatttc aacgtggccc atgggctggc atggtcatat
     4981 tacatcggat atctgcggct gatcctgcca gagctccagg cccggattcg aacttacaat
     5041 cagcattaca acaacctgct acggggtgca gtgagccagc ggctgtatat tctcctccca
     5101 ttggactgtg gggtgcctga taacctgagt atggctgacc ccaacattcg cttcctggat
     5161 aaactgcccc agcagaccgg tgaccgtgct ggcatcaagg atcgggttta cagcaacagc
     5221 atctatgagc ttctggagaa cgggcagcgg gcgggcacct gtgtcctgga gtacgccacc
     5281 cccttgcaga ctttgtttgc catgtcacaa tacagtcaag ctggctttag ccgggaggat
     5341 aggcttgagc aggccaaact cttctgccgg acacttgagg acatcctggc agatgcccct
     5401 gagtctcaga acaactgccg cctcattgcc taccaggaac ctgcagatga cagcagcttc
     5461 tcgctgtccc aggaggttct ccggcacctg cggcaggagg aaaaggaaga ggttactgtg
     5521 ggcagcttga agacctcagc ggtgcccagt acctccacga tgtcccaaga gcctgagctc
     5581 ctcatcagtg gaatggaaaa gcccctccct ctccgcacgg atttctcttg actcgagacc
     5641 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     5701 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     5761 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5821 tggaagggct aattcactcc caacgaagac aaaatcgtcg agagatgctg catataagca
     5881 gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5941 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6001 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6061 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     6121 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tataatagat ttagcaggaa
     6181 ttgaactagg agtggagcac acaggcaaag ctgcagaagt acttggaaga agccaccaga
     6241 gatactcacg attctgcaca tacctggcta atcccagatc ctaaggatta cattaagttt
     6301 actaacattt atataatgat ttatagttta aagtataaac ttatctaatt tactattctg
     6361 acagatatta attaatcctc aaatatcata agagatgatt actattatcc ccatttaaca
     6421 caagaggaaa ctgagaggga aagatgttga agtaattttc ccacaattac agcatccgtt
     6481 agttacgact ctatgatctt ctgacacaaa ttccatttac tcctcaccct atgactcagt
     6541 cgaatatatc aaagttatgg acattatgct aagtaacaaa ttaccctttt atatagtaaa
     6601 tactgagtag attgagagaa gaaattgttt gcaaacctga atagcttcaa gaagaagaga
     6661 agtgaggata agaataacag ttgtcattta acaagtttta acaagtaact tggttagaaa
     6721 gggattcaaa tgcataaagc aagggataaa tttttctggc aacaagacta tacaatataa
     6781 ccttaaatat gacttcaaat aattgttgga acttgataaa actaattaaa tattattgaa
     6841 gattatcaat attataaatg taatttactt ttaaaaaggg aacatagaaa tgtgtatcat
     6901 tagagtagaa aacaatcctt attatcacaa tttgtcaaaa caagtttgtt attaacacaa
     6961 gtagaatact gcattcaatt aagttgactg cagattttgt gttttgttaa aattagaaag
     7021 agataacaac aatttgaatt attgaaagta acatgtaaat agttctacat acgttctttt
     7081 gacatcttgt tcaatcattg atcgaagttc tttatcttgg aagaatttgt tccaaagact
     7141 ctgaaataag gaaaacaatc tattatatag tctcacacct ttgttttact tttagtgatt
     7201 tcaatttaat aatgtaaatg gttaaaattt attcttctct gagatcattt cacattgcag
     7261 atagaaaacc tgagactggg gtaattttta ttaaaatcta atttaatctc agaaacacat
     7321 ctttattcta acatcaattt ttccagtttg atattatcat ataaagtcag ccttcctcat
     7381 ctgcaggttc cacaacaaaa atccaaccaa ctgtggatca aaaatattgg gaaaaaatta
     7441 aaaatagcaa tacaacaata aaaaaataca aatcagaaaa acagcacagt ataacaactt
     7501 tatttagcat ttacaatcta ttaggtatta taagtaatct agccagatcc tctacgccgg
     7561 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     7621 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     7681 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     7741 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     7801 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
     7861 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     7921 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     7981 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     8041 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     8101 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8161 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8221 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8281 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8341 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8401 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8461 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8521 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8581 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8641 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8701 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8761 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8821 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8881 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8941 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9001 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9061 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9121 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9181 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9241 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9301 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9361 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9601 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9781 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9841 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9901 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9961 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    10021 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
    10081 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    10141 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    10201 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
    10261 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    10321 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    10381 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
    10441 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
    10501 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
    10561 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
    10621 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
    10681 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
    10741 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
    10801 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
    10861 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
    10921 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
    10981 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
    11041 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
    11101 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
    11161 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
    11221 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
    11281 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
    11341 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
    11401 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
    11461 cggatctttg tgaaggaacc ttacttctgt ggtgtg
//